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Why Dr. Rhonda Patrick Changed her Mind on Skipping Breakfast, Eating Bananas and More Hydration and energy levels Key Efficoency for Selenium IDE Materials and Joint International Research Efficienyc of Precision Chemistry and Glucose utilization efficiency Engineering, Feringa Utilizaiton Prize Scientist Joint Research Center, Glucose utilization efficiency Institute Selenium IDE Industrial Catalysis, School of Micronutrient-rich proteins and Molecular Uutilization, East Utilozation University of Science eficiency Technology, Shanghai, China E-mail: wangyanqin ecust. b Key Laboratory of Chemical Engineering, East China University of Science and Technology, ShanghaiChina. The efficient utilization of renewable glucose has enormous potential to reduce the excessive dependence on fossil resources, but remains challenging owing to the inevitable formation of undesirable humins and multistep processes. The formation of humins is markedly restrained with an entirely new pathway, i. glucose directly reacts with 2,4-pentanedione to afford fuel precursors via several chemical transformations, which is totally different from the traditional glucose-to-fructose-to-HMF way.

Glucose utilization efficiency -

Moreover, Aifm2 expression in C2C12 myotubes was not regulated by caffeine or the AMPK activator, AICAR, which are known activators of signaling pathways that facilitate exercise data not shown. This suggests that AIFM2 may be regulated by other signaling pathways and that it may also require in vivo context to modulate muscle contraction and exercise, selectively operating in EDL.

Further studies are necessary to identify upstream regulators of AIFM2 expression and mechanisms that confer EDL-selective regulation of glycolytic activity. EDL is composed of mostly fast-twitch glycolytic muscle fibers and expresses a higher level of glycolytic enzymes, such as phosphofructokinase-1 and lactate dehydrogenase, compared with soleus 29 ; thus, it is an ideal location for glycolysis.

Even though EDL has a small total mass, the absolute amount of glucose utilization is significantly greater in EDL compared with soleus Also, notably, EDL-specific reconstitution of Glut4 expression in Glut4 -null mice completely rescues their defect in insulin-stimulated glucose uptake These findings strongly support the idea that altered metabolism in EDL alone can shift whole-body glucose homeostasis.

In that sense, it is not entirely surprising to find that EDL-targeted OE and KD altered blood parameters of glucose during high-intensity exercise. However, we note that the nature of our gene delivery method via electroporation does not allow for achievement of complete muscle-depot specificity; thus, we cannot completely exclude a potential contribution from other muscle types.

Other than its role in mobility and exercise, skeletal muscle is an important metabolic tissue playing a key role in glycemic control. Patients with obesity and diabetes have defects in shifting from oxidative to glycolytic metabolism in response to insulin 32 , It has been suggested that reinforcing glucose utilization can serve as an adaptive response to improving metabolic dysregulation in diabetes conditions.

For example, inhibiting myostatin signaling leads to glycolytic muscle growth and improved glucose homeostasis Human studies showed that resistance training improves the metabolic profile of patients with diabetes by promoting the glycolytic program in fast-twitch muscles 35 , Similarly, a mouse genetic study showed that switching from oxidative to glycolytic metabolism due to transgenic expression of Baf60c in muscle confers protection from diet-induced insulin resistance Thus, it will be intriguing and important to test whether increased AIFM2 function at rest, even when dissociated with exercise, plays a beneficial role in the context of insulin resistance and glucose intolerance.

Overall, we found that skeletal muscle AIFM2 functions as an NADH oxidoreductase and plays a critical role in sustaining high-intensity exercise by supporting a high rate of glycolysis in skeletal muscles during exercise. A better understanding of the regulatory mechanisms of fuel utilization during exercise and diet-induced adaptation may shed light on novel therapeutic targets and strategies to improve exercise inefficiency.

The authors thank Dr. David Wasserman Vanderbilt University for sharing protocols for in vivo glucose uptake assay. Work was funded by National Institute of Diabetes and Digestive and Kidney Diseases R01 DK and Pilot and Feasibility P30DK [Stanford Diabetes Research Center] to S.

and R01 DK to H. Duality of Interest. No potential conflicts of interest relevant to this article were reported. Author Contributions. and S. supervised experiments, and S. co-wrote the manuscript. consulted on data analysis and interpretation. Experiments were carried out by H.

is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Sign In or Create an Account. Search Dropdown Menu. header search search input Search input auto suggest.

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Research Design and Methods. Article Information. Article Navigation. Metabolism June 30 AIFM2 Is Required for High-Intensity Aerobic Exercise in Promoting Glucose Utilization Hai P.

Nguyen ; Hai P. This Site. Google Scholar. Sneha Damal Villivalam ; Sneha Damal Villivalam. Byung Chul Jung ; Byung Chul Jung. Dongjoo You ; Dongjoo You. Frances Lin ; Frances Lin.

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Get Permissions. toolbar search Search Dropdown Menu. toolbar search search input Search input auto suggest. Data sets generated from the current study are available from the corresponding author. Figure 1. View large Download slide. Figure 2. Figure 3. Figure 4. contributed equally.

Interaction among skeletal muscle metabolic energy systems during intense exercise. Search ADS. van Loon. The effects of increasing exercise intensity on muscle fuel utilisation in humans. Cytosolic malate dehydrogenase activity helps support glycolysis in actively proliferating cells and cancer.

Creatine kinase and lactate dehydrogenase responses after different resistance and aerobic exercise protocols.

Exercise-stimulated glucose uptake - regulation and implications for glycaemic control. Aifm2, a NADH oxidase, supports robust glycolysis and is required for cold- and diet-induced thermogenesis. Damal Villivalam. A necessary role of DNMT3A in endurance exercise by suppressing ALDH1L1-mediated oxidative stress.

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We demonstrated that an L-lysine-producing E. This approach should increase the efficiency of industrial fermentation using E. coli and would facilitate full utilization of valuable carbohydrate resources.

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Download references. We are grateful to Dr. for providing technical advice. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Kagamiyama, Higashi-Hiroshima-shi, Hiroshima, , Japan. You can also search for this author in PubMed Google Scholar.

Correspondence to Kenji Abe. This article does not contain any studies with human participants performed by any of the authors. Open Access This article is distributed under the terms of the Creative Commons Attribution 4. Reprints and permissions. Abe, K. Engineering of Escherichia coli to facilitate efficient utilization of isomaltose and panose in industrial glucose feedstock.

Appl Microbiol Biotechnol , — Download citation. Received : 29 July Revised : 27 October Accepted : 26 November Published : 08 December Issue Date : March Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative. Download PDF. Abstract Industrial glucose feedstock prepared by enzymatic digestion of starch typically contains significant amounts of disaccharides such as maltose and isomaltose and trisaccharides such as maltotriose and panose.

Metabolic engineering of carbohydrate metabolism systems in Corynebacterium glutamicum for improving the efficiency of l-lysine production from mixed sugar Article Open access 18 February Engineering Escherichia coli for highly efficient production of lacto-N-triose II from N-acetylglucosamine, the monomer of chitin Article Open access 08 October Engineering Escherichia coli for Direct Production of 1,2-Propanediol and 1,3-Propanediol from Starch Article 09 September Use our pre-submission checklist Avoid common mistakes on your manuscript.

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Similar patterns of myocardial metabolism and perfusion in patients with type 2 diabetes and heart disease of ischaemic and non-ischaemic origin. Download references. Department of Medical and Surgical Sciences, University Magna Graecia of Catanzaro, Viale Europa, , Catanzaro, Italy.

Research Center for the Prevention and Treatment of Metabolic Diseases CR METDIS , University Magna Graecia of Catanzaro, Catanzaro, Italy. Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, Catanzaro, Italy. Department of Clinical and Molecular Medicine, University of Rome-Sapienza, , Rome, Italy.

You can also search for this author in PubMed Google Scholar. ES researched and analyzed data and wrote and edited the manuscript. FC and AP performed cardiac PET scans. SM performed the echocardiographic exams. PVi and PHG analyzed the data from the cardiac PET scans. TVF, MP, AS researched data and reviewed the manuscript.

PVe, GLC and FA contributed to the discussion and reviewed the manuscript. GS designed the study, analyzed the data, and wrote and reviewed the manuscript. All authors have read and approved the final manuscript. GS is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

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Reprints and permissions. Succurro, E. et al. Impaired insulin-stimulated myocardial glucose metabolic rate is associated with reduced estimated myocardial energetic efficiency in subjects with different degrees of glucose tolerance.

Cardiovasc Diabetol 22 , 4 Download citation. Received : 27 September Accepted : 28 December Published : 09 January Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article.

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Selenium IDE Cell Selenium IDE volume 21Endurance nutrition for recovery number: Cite this article. Metrics details. Glucose utilization efficiency modification of Glicose import capacity is an engineering utilizatipn that has lGucose shown to improve the characteristics of Escherichia Glucose utilization efficiency as a microbial factory. A reduction in glucose import capacity can have a positive effect on production strain performance, however, this is not always the case. In this study, E. coli W and a group of four isogenic derivative strains, harboring single or multiple deletions of genes encoding phosphoenolpyruvate:sugar phosphotransferase system PTS -dependent transporters as well as non-PTS transporters were characterized by determining their transcriptomic response to reduced glucose import capacity.